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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is validating a new multiplex PCR assay for the simultaneous detection of several gastrointestinal pathogens, including Salmonella, Shigella, and Campylobacter. To ensure the accuracy and reliability of the assay, it is crucial to include appropriate controls. Which of the following sets of controls would be MOST comprehensive?

A) Positive controls for each target organism, a negative control without template DNA, and an internal amplification control.
B) Positive controls for each target organism, a negative control containing non-target DNA, and a process control to monitor nucleic acid extraction.
C) Positive controls for each target organism and a negative control without any template DNA.
D) Positive controls for each target organism, a negative control without template DNA, an internal amplification control, and a process control to monitor nucleic acid extraction.

2. A microbiology laboratory isolates a Gram-negative rod from a wound infection. The organism is oxidase- negative, ferments lactose on MacConkey agar, and produces indole. The MOST likely identification is:

A) Serratia marcescens
B) Klebsiella pneumoniae
C) Proteus mirabilis
D) Escherichia coli

3. A patient with a history of solid organ transplantation develops diarrhea and abdominal cramps. Stool studies are negative for bacterial pathogens and C. difficile toxins. Colonoscopy reveals characteristic cytomegalic inclusion bodies in colonic biopsies. The MOST likely causative agent is:

A) Cytomegalovirus (CMV)
B) Norovirus
C) Adenovirus
D) Rotavirus

4. A clinical microbiology laboratory is implementing a new quality control procedure for blood culture media.
To ensure the media supports the growth of a wide range of clinically significant organisms, they should include:

A) Only control strains that are commonly found as contaminants.
B) A diverse panel of fastidious and non-fastidious bacteria and yeasts.
C) Only antibiotic-resistant control strains.
D) Only Gram-positive and Gram-negative control strains.

5. A Gram stain of cerebrospinal fluid (CSF) from a 2-year-old child with meningitis shows small, pleomorphic Gram-negative coccobacilli. Culture on chocolate agar yields growth, but there is no growth on standard 5% sheep blood agar unless streaked near a colony of Staphylococcus aureus. This phenomenon indicates a requirement for which growth factor(s)?

A) Factor X (hemin) only
B) Both Factor X and Factor V
C) Cysteine and pyridoxal
D) Factor V (NAD) only

Solutions: