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American Society of Microbiology ABMM Sample Questions:

1. A research laboratory is investigating the mechanisms of antiviral drug resistance. They are studying a herpes simplex virus strain that has developed resistance to acyclovir. The MOST common mechanism of acyclovir resistance in HSV involves mutations in the gene encoding:

A) Viral thymidine kinase
B) Viral glycoprotein D
C) Viral protease
D) Viral DNA polymerase

2. A clinical microbiology laboratory is investigating a cluster of Clostridium difficile infections in a hospital.
Which of the following methods is MOST appropriate for determining if the isolates from different patients are related?

A) Enzyme immunoassay for C. difficile toxins
B) Molecular typing (e.g., PCR ribotyping)
C) Gram staining and morphology
D) Antimicrobial susceptibility testing

3. A Klebsiella pneumoniae isolate from a bloodstream infection shows the following susceptibility profile:
Meropenem (MIC = 8 µg/mL, R), Imipenem (MIC = 16 µg/mL, R), Ertapenem (MIC = 4 µg/mL, R), Ceftazidime/avibactam (MIC = 1 µg/mL, S), Aztreonam (MIC = 32 µg/mL, R). Which resistance mechanism is most strongly suggested by this pattern?

A) Extended-Spectrum Beta-Lactamase (ESBL) production alone
B) Metallo-beta-lactamase (MBL) production
C) Klebsiella pneumoniae Carbapenemase (KPC) production
D) AmpC beta-lactamase hyperproduction coupled with porin loss

4. A patient with a history of travel to a tropical region presents with cyclical fevers and chills.Peripheral blood smear reveals the presence of merozoites and schizonts within erythrocytes. The absence of crescent-shaped gametocytes makes Plasmodium falciparum less likely. The MOST probable species is:

A) Plasmodium vivax
B) Plasmodium knowlesi
C) Plasmodium malariae
D) Plasmodium ovale

5. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Klebsiella pneumoniae isolate. The isolate is resistant to multiple beta-lactam antibiotics, and further testing reveals the presence of a carbapenemase enzyme. The MOST clinically significant carbapenemase in Klebsiella pneumoniae is often:

A) AmpC beta-lactamase
B) Extended-spectrum beta-lactamase (ESBL)
C) Klebsiella pneumoniae carbapenemase (KPC)
D) Metallo-beta-lactamase (MBL)

Solutions: